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KMID : 0880220130510030323
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Journal of Microbiology
2013 Volume.51 No. 3 p.323 ~ p.328
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A novel retron of Vibrio parahaemolyticus is closely related to retron-Vc95 of Vibrio cholerae
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Toshi Shimamoto
Tadashi Shimamoto
Ashraf M. Ahmed
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Abstract
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Some bacteria produce a satellite RNA-DNA complex termed msDNA, multicopy single-stranded DNA. In this report, msDNA from Vibrio parahaemolyticus, a cause of acute gastroenteritis, was identified and named msDNA-Vp96. The retron element containing the ret gene, encoding the reverse transcriptase (RT) that is responsible for msDNA production, was cloned and characterized. Comparison of msDNA-Vp96 and msDNA-Vc95, from Vibrio cholerae, showed a high level of sequence similarity. We exchanged the two ret genes to examine whether msDNA was produced by the RT from different sources. We found that RT-Vp96 of V. parahaemolyticus was able to synthesize msDNA-Vc95 of V. cholerae and vice versa. To the best of our knowledge, this is the first report that RT from different bacterial species can synthesize msDNA.
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KEYWORD
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reverse transcriptase, msDNA, pathogenic bacteria, vibrios
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